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KMID : 1134820140430010060
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Journal of the Korean Society of Food Science and Nutrition
2014 Volume.43 No. 1 p.60 ~ p.66
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Antioxidant Activity and Protective Effects of Extracts from Chrysanthemum boreale on t-BHP Induced Oxidative Stress in Chang Cells
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Kim Yon-Suk
Hwang Jin-Woo
Park Pyo-Jam
Jeong Jae-Hyun
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Abstract
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The aim of this study was to evaluate the antioxidant activity and protective effect of extracts from the stems and leaves of Chrysanthemum boreale (CBSL) on t-BHP induced oxidative stress in human liver cells (Chang cells). Antioxidant activities in the extracts were determined for various radical scavenging activities including ferric reducing antioxidant power, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity, and oxygen radical absorbance capacity (ORAC). CBSL showed a very good scavenging effect of DPPH radical (IC50 0.009± 0.002 mg/mL), alkyl radical (IC50 0.004±0.001 mg/mL), and hydroxyl radical (IC50 6.742±0.152 mg/mL). CBSL also showed a strong antioxidant effect in the ORAC assay. In the MTT assay on human liver cells (Chang cells), the extracts showed protective effects by increasing cell viability, decreasing ROS, and restoring mitochondria membrane potential upon t-BHP induced oxidative stress. Our findings suggest that CBSL extracts are a potential therapeutic with protective antioxidant effects upon oxidative stress.
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KEYWORD
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antioxidant activity, t-BHP, Chrysanthemum boreale L., oxidative stress
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